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. Author manuscript; available in PMC: 2017 Jan 16.
Published in final edited form as: ACS Nano. 2015 Jun 2;9(7):6683–6695. doi: 10.1021/acsnano.5b00690

Figure 3.

Figure 3

Construction of bicistronic vector with tubb3 promoter driven reporter gene. (A) Gel electrophoresis of the cloned tubb3 promoter from three independent brain tissue extracts. The #2 clone was used in the entire study. (B) Fluorescence image of lentivirus transduced NE-4C NSCs with tubb3 promoter driven fLuc reporter gene and independent EF1α driven GFP reporter gene. Scale bar 50 µm. (C) Confocal laser scanning microscopy imaging of NE-4C NSC differentiation by 100 nM RA-loaded nanovehicle over 8 days. βIII tubulin was stained by Cy5.5-conjugated secondary antibody. The GFP and mCherry were endogenously expressed fluorescence proteins in transduced NSCs. Scale bar = 50 µm. (D) Bioluminescence imaging (BLI) of tubb3 promoter driven fLuc expression in transduced differentiating NSCs stimulated by 100 nM RA-loaded nanovehicle over 8 days. (E) Analysis of BLI results (n = 3).