Figure 3.

v6‐PEG efficiently blocks cell scattering and HGF/Met signaling. A) ASs6 cells treated for 30 min with 150 × 10⁻⁹ m of v6‐peptide (A = synthesized by ourselves, B = synthesized by Bachem, Bubendorf, Switzerland), v6‐PEG, v6 control, or v6‐PEG control were induced with HGF (10 ng mL⁻¹) for 5 min. Subsequently, Met and Erk phosphorylation were determined by western‐blot analysis. p‐Met and p‐Erk levels were normalized to total Met and Erk levels, respectively. Quantification of the fold induction of activation is shown in the diagram. B) HT29 cells grown in the absence (control) or presence of HGF with or without v6‐peptide, v6‐PEG, v6 control, or v6‐PEG control were visualized by phase contrast microscopy. Magnification, 40×. The table reflects the scattering ability of HT29 cells in the absence or presence of the respective peptides.