Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 12;12:447–457. doi: 10.2147/IJN.S116749

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 Keller et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

Behavior of osteoblasts seeded on 3D nanofibrous PCL as single cells or as microtissues.

Notes: Fluorescence confocal microscopy visualization (A, B, D, E) of OBs (A, B) and OB-MTs (D, E) grown onto the 3D PCL, and visualized in blue with DAPI (DNA staining) and green with poly-lysine-FITC (nanofibers staining) after 3 days (A and D) and 21 days (B and E) in vitro. (C, F) AR of bone induction and mineralization on the 3D thick PCL electrospun nanofiber seeded with OB single cells (C) and OB-MTs (F) after 21 days. Scale bars =100 µm (A, B, D, E), and 1 mm (C, F).

Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; AR, Alizarin Red staining; FITC, fluorescein isothiocyanate; OB, human primary osteoblast; OB-MTs, human primary osteoblast microtissues; PCL, poly(ε-caprolactone); PLL, poly-lysine.