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. 2017 Jan 12;12:473–486. doi: 10.2147/IJN.S123625

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© 2017 Wang et al. This work is published and licensed by Dove Medical Press Limited

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The outline of MCDA combined with LFB.

Notes: (A) Schematic depiction of the new cross primer (CP1*) and amplification primer (D1*). (B) Outline of MCDA with CP1* and D1*. (C) Schematic illustration of the principle of LFB for visualization of MCDA amplicons. (D) Interpretation of the results: I, positive (two red lines at the readout area) and II, negative (only the control line zone showed a red band). C1*, 5′-labeled with Dig when used in MCDA-LFB assay; CP1*, 5′-labeled with biotin when used in MCDA-LFB assay.

Abbreviations: BSA, bovine serum albumin; Dig, digoxigenin; GNPs, gold nanoparticles; LFB, lateral flow biosensor; MCDA, multiple cross displacement amplification; NC, nitrocellulose; SA, streptavidin.