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. 2017 Jan 12;12:473–486. doi: 10.2147/IJN.S123625

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© 2017 Wang et al. This work is published and licensed by Dove Medical Press Limited

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Verification and detection of Listeria monocytogenes-MCDA products.

Notes: (A) Reaction products of L. monocytogenes-MCDA assay were visually detected by observation of the color change. (B) Agarose gel electrophoresis of L. monocytogenes-MCDA products was shown. (C) LFB applied for visual detection of L. monocytogenes-MCDA products. Tube 1/lane 1/strip 1, positive amplification of L. monocytogenes strain (EGD-e); tube 2/lane 2/strip 2, negative control of Listeria ivanovii strain (ATCCBAA-678); tube 3/lane 3/strip 3, negative control of Salmonella strain (ICDC-NPSa001); tube 4/lane 4/strip 4, blank control (DW). Lane M, DNA maker DL 100.

Abbreviations: DW, double distilled water; LFB, lateral flow biosensor; MCDA, multiple cross displacement amplification.