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. 2017 Jan 12;12:473–486. doi: 10.2147/IJN.S123625

Figure 6.

Figure 6

Analytical sensitivity of MCDA-LFB assay using serially diluted genomic DNA with Listeria monocytogenes strain EGD-e.

Notes: A total of 4 monitoring methods, including LFB (A), colorimetric indicator (FD) (B), gel electrophoresis (C), and real-time turbidity (D) were applied for analyzing the amplification products. The serial dilutions (10 ng, 10 pg, 10 fg, 1 fg, and 0.1 fg) of target templates were subjected to standard MCDA reactions. Strips (A)/tubes (B)/lanes (C)/turbidity signals (D) 1–8 represented the DNA levels of 10 ng, 10 pg, 10 fg, 1 fg, and 0.1 fg per reaction, negative control (10 pg of Listeria ivanovii genomic DNA), negative control (10 pg of Salmonella genomic DNA) and blank control (DW). The genomic DNA levels of 10 ng, 10 pg, and 10 fg per reaction produced the positive reactions.

Abbreviations: BC, blank control; DW, double distilled water; FD, fluorescent detection reagent; LFB, lateral flow biosensor; MCDA, multiple cross displacement amplification.