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. 2017 Jan 12;12:473–486. doi: 10.2147/IJN.S123625

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© 2017 Wang et al. This work is published and licensed by Dove Medical Press Limited

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The optimal duration of time required for MCDA-LFB assay.

Notes: Four different reaction times (A) 10 min, (B) 15 min, (C) 20 min, and (D) 25 min were tested and compared at 61°C. Strips 1–8 represent DNA levels of 10 ng of Listeria monocytogenes templates, 10 pg of L. monocytogenes templates, 10 fg of L. monocytogenes templates, 1 fg of L. monocytogenes templates, 0.1 fg L. monocytogenes templates per tube, negative control (Listeria ivanovii, 10 pg per reaciton), negative control (Salmonella, 10 pg per reaction), and blank control (DW). The best sensitivity was seen when the amplification lasted for 20 min (C).

Abbreviations: DW, double distilled water; LFB, lateral flow biosensor; MCDA, multiple cross displacement amplification.