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. 2017 Jan 12;12:473–486. doi: 10.2147/IJN.S123625

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© 2017 Wang et al. This work is published and licensed by Dove Medical Press Limited

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The specificity of MCDA-LFB assay for different strains.

Notes: The MCDA reactions were carried out using different genomic DNA templates and were monitored by means of visual format. Biosensors 1–12, Listeria monocytogenes strains of serovar 1/2a (ATCC51772), 3a (ATCC51782), 1/2c (Slcc2372), 3c (Slcc2479), 1/2b (Slcc2755), 3b (Slcc2540), 7 (NCTC10890), 4a (ATCC19114), 4c (ATCC19116), 4b (ATCC19115), 4d (ATCC19117), and 4e (ATCC19118); biosensor 13–17, Listeria ivanovii (ATCCBAA-678), Listeria innocua (ATCCBAA-680), Listeria grayi (ATCC25402), Listeria seeligeri (ATCC35897), and Listeria welshimeri (ATCC35897); biosensors 18–39, Bacillus cereus, Enteropathogenic Escherichia coli, Enterotoxigenic E. coli, Enteroaggregative E. coli, Enteroinvasive E. coli, Enterohemorrhagic E. coli, Plesiomonas shigelloides, Shigella boydii, Shigella sonneri, Shigella flexneri, Enterobacter cloacae, Enterococcus faecalis, Yersinia enterocolitica, Streptococcus pneumonia, Aeromonas hydrophil, Vibrio vulnificus, Vibrio fluvialis, Vibrio parahaemolyticus, Proteus vulgaris, Streptococcus bovis, Klebsiella pneumonia, and Salmonella enteric; and biosensor 40, blank control (DW).

Abbreviations: ATCC, American Type Culture Collection; DW, double distilled water; LFB, lateral flow biosensor; MCDA, multiple cross displacement amplification.