Table 1.
The primers used in this study
| Primers | Sequences and modifications (5′–3′) | Length |
|---|---|---|
| F1 | AAGGACTTTCGCAAGAAGA | 19 nt |
| CP1 | AGGAACTGACAGTCCTTGCTATCAAACTGAATGTGGTGC | 39 mer |
| CP1* | Biotin-AGGAACTGACAGTCCTTGCTATCAAACTGAATGTGGTGC | 39 mer |
| C1 | AGGAACTGACAGTCCTTGCT | 20 nt |
| C1* | Dig-AGGAACTGACAGTCCTTGCT | 20 nt |
| D1 | CCCATTTAGAGATTGTTTGTC | 21 nt |
| R1 | GGAGATTAACATATCGGAATC | 21 nt |
| R2 | GAAGTGCTTGAAACACCAGT | 20 nt |
| D2 | CAAAGGTTGATGATGTAAAAGC | 22 nt |
| C2 | CACTTTGCTTGGAGAAACTGTTATG | 25 nt |
| CP2 | CACTTTGCTTGGAGAAACTGTTATGAAGTTTATAATCTCCAGTTTTTCGG | 50 mer |
| F2 | GGCGGTCTTTCTTTGAGC | 18 nt |
Notes: C1*, 5′-labeled with Dig when used in MCDA-LFB assay; CP1*, 5′-labeled with biotin when used in MCDA-LFB assay.
Abbreviations: LFB, lateral flow biosensor; MCDA, multiple cross displacement amplification; mer, monomeric; nt, nucleotide.