Skip to main content
. 2016 Dec 23;50(2):373–380. doi: 10.3892/ijo.2016.3819

Figure 3.

Figure 3

Nek2A is regulated by Hh signaling. (A) mRNA levels of Nek2A in GANT61 treated H4 cells. H4 cells were treated with GANT61 for 0, 12 and 24 h, respectively. Total mRNA was extracted and quantified by real-time PCR. Error bars represent the standard deviation of three independent experiments. (B) Protein levels of Nek2A in GANT61 treated cells. H4 cells were treated with GANT61 for 0, 12 and 24 h, respectively. Total proteins were extracted and Nek2A was detected by western blotting with monoclonal antibody. (C) mRNA levels of Nek2A in purmorphamine treated H4 cells. Total mRNA was extracted and quantified by real-time PCR. Error bars represent the standard deviation of three independent experiments. **P<0.01 compared with the control groups.