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. 2016 Jul 24;7(32):50914–50926. doi: 10.18632/oncotarget.10812

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Copyright: © 2016 Choi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Functional correlation and interaction of IL2RG signaling using the Pathway Studio software (Ariadne Genomics Inc.; Rockville, MD, USA). All genes are shown by their gene symbols. Direct and indirect regulation are indicated by colored lines and dotted gray lines, respectively. B. and C. RT-qPCR validation of IL2RG upstream (B)- and downstream (C)-related gene expression proposed by Studio Pathway Analysis. D. A western blot analysis of AKT, pAKT, STAT5, pSTAT5, STAT3 and p STAT3 was performed in thymus of WT and mIL2RG^+/Δ69-368 KO pigs. B actin mRNA expression, which used as an internal positive control in both of WT and mIL2RG^+/Δ69-368 KO pig-derived thymus, was found to be similar. The data are representative of three experiments.