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. 2016 Jun 13;7(32):51223–51236. doi: 10.18632/oncotarget.9981

Figure 4. Akt1 promote ESCC progression in vitro.

Figure 4

(A) Representative images of western blot analysis of the Akt1 protein level in ESCC cells after transfection. The expression level of Akt1 protein was significantly improved in cells transfected with Akt1 vector and depressed in cells transfected with siRNA (B) Representative images (left) and quantifiation (right) of ESCC cell colonies, as determined by colony formation assay after transfection. The over-expression of Akt1 protein significantly improved the colony formation number of ESCC cell lines. Conversly, down-regulation of Akt1 protein significantly reduced the colony formation number of ESCC cell lines (C) Flow cytometry analysis of ESCC cells after transfection. Cell cycle analysis revealed that Akt1 has influence the proliferation of ESCC cells by regulating its cell cycle. (D) Representative images (left) and quantifiation (right) of migratory and invasive cells per field after transfection. miR-495 significantly depressed the migratory or invasive cells per field.