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. 2016 Jun 13;7(32):51223–51236. doi: 10.18632/oncotarget.9981

Figure 5. Akt1 are involved in the role of miR-495 in regulation of ESCC cell activity.

Figure 5

(A) Representative images of western blot analysis of the Akt1 protein level in ESCC cells after transfection. The expression level of Akt1 protein in cells transfected with plasmid were higher than that of the negative control, even with suppression by miR-495. (B) Representative images (left) and quantifiation (right) of ESCC cell colonies, as determined by colony formation assay after transfection. The suppressive effects of miR-495 on cell proliferation has been partially attenuated by the expression of Akt1. (C) Flow cytometry analysis of ESCC cells after transfection. The effects of miR-495 on cell cycle has been partially abolished by the expression of Akt1. (D) Representative images (left) and quantifiation (right) of migratory and invasive cells per field after transfection. The effects of miR-495 on cell migration and invasion has been partially abolished by the expression of Akt1.