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. 2016 Jul 8;7(32):51503–51514. doi: 10.18632/oncotarget.10491

Figure 2. Reduced expression of HOXA9 by inhibition of integrin α2.

Figure 2

(A) Total cell lysates were extracted from two MDA-MB-231 cells variants that were transduced with lentiviral particles expressing integrin α2-specific Mission shRNA (ITGα2KD) or a matching control shRNA (CTL). The protein levels of integrin α2 were measured using immunoblots. (B) Total cell RNA was extracted from the ITGα2KD and CTL variants on day 6 of lrECM 3D culture. The mRNA levels of HOXA9 were measured using qRT-PCR. A fold change of HOXA9 in ITGα2KD cells over CTL cells was obtained by normalizing to the housekeeping gene RPLP0 and setting the values from CTL cells to one. When presented, means and standard deviations were obtained from three independent experiments. * and *** indicate a P value < 0.05 and 0.001, respectively.