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. 2016 Jul 9;7(32):51569–51580. doi: 10.18632/oncotarget.10514

Figure 1. Tris DBA decreases proliferation and colony forming ability of pancreatic carcinoma cells.

Figure 1

(A) Pan02 cells were treated for 24 h with the indicated concentrations of tris DBA or control vehicle (DMSO). Cells were counted 24 h after treatment and average of quadruplicate experiments ± S.D. represented. (*) p < 0.05; (**) p < 0.005 (***) p < 0.0005 (Student's t test) (B) Pan02 were plated at low density and treated 24 h after seeding with the indicated concentrations of tris DBA or vehicle control. Number of colonies visualized with crystal violet 6 days after treatment was quantified and represented as average ± S.D. of triplicate experiments. (*) p < 0.001; (**) p < 0.00001 (Student's t test). (C) Representative images of crystal violet-stained colonies after treatment of Pan02 cells with the indicated concentrations of tris DBA. (D) PANC1 were plated at low density and treated 24 h after seeding with the indicated concentrations of tris DBA or vehicle control. Number of colonies visualized with crystal violet 6 days after treatment was quantified and represented as average ± S.D. of triplicate experiments. (*) p < 0.001; (**) p < 0.0001 (Student's t test). (E) Representative images of crystal violet-stained colonies after treatment of PANC1 cells with the indicated concentrations of tris DBA.