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. 2016 Jul 9;7(32):51569–51580. doi: 10.18632/oncotarget.10514

Figure 7. SFK activity is not required for cilia formation in Pan02 cells.

Figure 7

(A) Pan02 cells were treated tris-DBA (1 μM) or the SFK inhibitors Su6656 (5 μM) or Su11333 (1 μM) for 6 hours. Cells were stained with acetylated tubulin to detect cilia. Percent of cells forming cilia was quantified in at least 200 cells per condition in three independent experiments and represented as average ± S.E.M. (*) p < 0.001 (Student's t-test). (B) Pan02 cells were treated with tris-DBA (1 μM) or the SFK inhibitors Su6656 (5 μM) or Su11333 (1 μM) for 6 hours. Cell extracts were processed for immunoblotting with p(Y705) Stat3, total Stat3 and Tubulin antibodies to assess the effectiveness of SFK inhibition.