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. 2016 Jul 12;7(32):51626–51639. doi: 10.18632/oncotarget.10539

Figure 6. Dasatinib and PKI-587 inhibit tumor growth of 138T muscle-invasive bladder cancer with EGFR amplification and PTEN deletion in the patient-derived xenograft (PDX) tumor mouse model.

Figure 6

(A) Measurement of subcutaneous xenograft tumor size after treatment with dasatinib or PKI-587. **P < 0.01. ***P < 0.001. PDX tumors were treated with dasatinib (15 mg/kg, n = 8) and PKI-587 (25 mg/kg, n = 8) for 17 days, harvested, and processed for immunoblots. (B) Measurement of mouse body weight after treatment with dasatinib or PKI-587 showing no toxicity from the drug therapy in the PDX mouse model. Body weights were measured after treatment with dasatinib or PKI-587 for 17 days. (C) Immunoblots of p-SRC (Tyr416), p-p70S6K (Thr389) and p-AKT (Ser473) in xenograft tumors from (A). (D) Cell proliferation and apoptosis were measured immunohistochemically (IHC) by performing Ki-67 and TUNEL staining using PDX tissues treated with dasatinib or PKI-587 (left). Scale bar, 100 μm. Representative bar graphs of the IHC images (right), **P < 0.01, ***P< 0.001 (n = 5 fields; means ± SD, analyzed by two-tailed paired t-test).