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. 2016 Jun 16;7(32):51733–51746. doi: 10.18632/oncotarget.10104

Figure 3. Sema3A promoted tumor growth and metastasis by recruiting macrophages (nude mouse model).

Figure 3

A. Recombinant human Sema3A recruited macrophages from HCC patients. This response was abrogated by anti-Sema3A antibody. *p<0.05; **p<0.01 compared with control; #P < 0.05 when compared with control antibody (Ab). B. Transwell assay and chemotactic migration of macrophages was determined after exposure to conditioned medium derived from different HCC cells. When we treated the CM of HCC cells with a high level of Sema3A with anti-Sema3A antibody, chemotactic response of macrophage decreased. Results shown are mean (±SD) values from three independent experiments, *p<0.05; **p<0.01 compared with control; #P < 0.05 when compared with control Ab. C. Representative bioluminescence images of orthotopic tumor at day 35. The color scale bar depicts the photon flux emitted from these tumors, n=8. D. Growth curves of tumors in xenograft nude mouse models, assessed by bioluminescent signals, n=8, *p<0.05; **p<0.01. E. Representative views of H&E stain results of metastatic nodules (lung tissue) from each group, n=8. F. Representative images of tumor samples from xenograft nude mouse models stained with F4/80. Magnification, ×200.