Table 2. Univariate and multivariate analyses of prognostic factors in HCC (cohort 3, n =368).
| Variable | TTR | OS | ||
|---|---|---|---|---|
| HR (95% CI) | P | HR (95% CI) | P | |
| Univariate analysis | ||||
| Age, year (≤50 versus >50) | 1.239(0.939-1.634) | 0.130 | 1.036(0.765-1.402) | 0.820 |
| Sex (female versus male) | 1.422(0.977-2.069) | 0.066 | 1.115(0.749-1.659) | 0.593 |
| HBsAg (negative versus positive) | 1.378(0.915-2.076) | 0.124 | 1.160(0.749-1.798) | 0.506 |
| AFP, ng/ml (≤20 versus >20) | 1.340(1.016-1.767) | 0.038 | 1.654(1.203-2.272) | 0.002 |
| GGT, U/L (≤54 versus >54) | 1.702(1.305-2.220) | 0.000 | 1.832(1.360-2.468) | 0.000 |
| Liver cirrhosis (no versus yes) | 1.643(1.098-2.459) | 0.016 | 1.459(0.934-2.280) | 0.097 |
| Tumor size, cm (≤5 versus >5) | 1.515(1.162-1.976) | 0.002 | 1.925(1.435-2.582) | 0.000 |
| Tumor number (single versus multiple) | 1.652(1.128-2.418) | 0.010 | 1.790(1.195-2.681) | 0.005 |
| Microvascular invasion (no versus yes) | 1.767(1.346-2.319) | 0.000 | 1.821(1.349-2.457) | 0.000 |
| Tumor encapsulation (complete versus none) | 1.289(0.991-1.678) | 0.059 | 1.266(0.944-1.697) | 0.115 |
| Tumor differentiation (I + II versus III + IV) | 1.451(1.082-1.946) | 0.013 | 1.757(1.282-2.406) | 0.000 |
| TNM stage (I versus II III) | 1.738(1.333-2.267) | 0.000 | 1.986(1.481-2.663) | 0.000 |
| Sema3A (low versus high) | 1.663(1.274-2.172) | 0.000 | 2.072(1.531-2.805) | 0.000 |
| Intratumor CD68 (low versus high) | 1.956(1.493-2.563) | 0.000 | 2.223(1.639-3.015) | 0.000 |
| Intratumor CD163 (low versus high) | 1.923(1.469-2.519) | 0.000 | 2.122(1.567-2.874) | 0.000 |
| Combination of Sema3A and CD163* | 0.000 | 0.000 | ||
| I versus II | 1.602(1.113-2.306) | 0.011 | 1.885(1.239-2.867) | 0.003 |
| II versus III | 1.323(0.948-1.847) | 0.099 | 1.387(0.959-2.006) | 0.082 |
| I versus III | 2.125(1.558-2.899) | 0.000 | 2.646(1.858-3.767) | 0.000 |
| Multivariate analysis | ||||
| AFP, ng/ml (≤20 versus >20) | 1.101(0.826-1.469) | 0.512 | 1.328(0.955-1.847) | 0.091 |
| GGT, U/L (≤54 versus >54) | 1.628(1.239-2.141) | 0.000 | 1.722(1.266-2.342) | 0.001 |
| Liver cirrhosis (no versus yes) | 1.597(1.062-2.401) | 0.024 | NA | NA |
| Tumor size, cm (≤5 versus >5) | 1.217(0.919-1.612) | 0.171 | 1.476(1.086-2.007) | 0.013 |
| Tumor number (single versus multiple) | 1.756(0.993-3.108) | 0.053 | 1.347(0.703-2.580) | 0.369 |
| Microvascular invasion (no versus yes) | 1.943(0.952-3.967) | 0.068 | 1.030 (0.480-2.212) | 0.939 |
| Tumor differentiation (I + II versus III + IV) | 1.405(1.038-1.901) | 0.028 | 1.671(1.208-2.311) | 0.002 |
| TNM stage (I versus II III) | 0.722(0.335-1.557) | 0.406 | 1.375(0.601-3.147) | 0.451 |
| Sema3A (low versus high) | 1.577(1.197-2.078) | 0.001 | 1.912(1.401-2.610) | 0.000 |
| Intratumor CD68 (low versus high) | 1.698(1.282-2.249) | 0.000 | 1.968(1.441-2.689) | 0.000 |
| Intratumor CD163 (low versus high) | 1.687(1.277-2.229) | 0.000 | 1.876(1.375-2.561) | 0.000 |
| Combination of Sema3A and CD163* | 0.000 | 0.000 | ||
| I versus II | 1.506(1.032-2.197) | 0.034 | 1.606(1.037-2.486) | 0.034 |
| II versus III | 1.386(0.987-1.947) | 0.059 | 1.432(0.986-2.081) | 0.060 |
| I versus III | 1.964(1.430-2.698) | 0.000 | 2.296(1.593-3.309) | 0.000 |
Cox proportional hazards regression model.
*
I, Sema3Alow/CD163low; II, Sema3Alow/CD163high and Sema3Ahigh/CD163low; III, Sema3Ahigh/CD163high. AFP, alpha-fetoprotein; GGT, gamma glutamyl transferase; TNM, tumor-node-metastasis.
HR, hazard ratio; CI, confidential interval; NA, not adopted.