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. 2016 Jun 16;7(32):51784–51814. doi: 10.18632/oncotarget.10108

Figure 7. E2F3 proto-oncogene is a target of miR-377-3p at specific 3′-UTR sites.

Figure 7

A. miR-377-3p is lowly expressed in primary human lung cancer tissues (n=96) and NSCLC cell lines. B. Scatter plots showing the inverse association between miR-377-3p level and E2F3 mRNA expression in primary human lung cancer tissues (n=96). C. E2F3 is up-regulated in primary human lung cancer tissues (n=96). D. Left pmiR-RB-REPORT™ dual-luciferase reporter vector. Middle The 3′-UTR of E2F3 harbors two miR-377-3p cognate sites. Right Relative luciferase activity of reporter plasmids carrying wild-type or mutant E2F3 3′-UTR in A549 and H1299 cells co-transfected with negative control (NC) or miR-377-3p mimic. E. miR-377-3p suppressed the protein expression of E2F3. Assays were performed in triplicate. *P < 0.05. Means ± SEM are shown. Statistical analysis was conducted using student t-test.