Skip to main content
. 2016 Jun 17;7(32):51815–51828. doi: 10.18632/oncotarget.10122

Figure 2. Replication-deficient adenoviruses amplified in HUMSC.LentiR.E1A and were eventually released from the cells.

Figure 2

A. The mRNA expression level of E1A at indicating time points after LentiR.E1A infection. B. The total concentration (intracellularly and in the supernatant) of viral DNA was measured at different time points in in three independent samples of HUMSC sequentially infected by Ad-Track and LentiR.E1A / LentiR. The start of LentiR.E1A infection was set as 0 h. C. The intracellular and supernatant concentration of viral DNA were detected at different time points respectively. D. Electron micrographs show viral particles in the HUMSC sequentially infected by Ad-Track and LentiR.E1A. Adenovirus particles were shown by the black arrow. Left panel: at low magnification; Right panel: at high magnification. E. The infection efficiency of HepG2 was detected by FACS 72 h after co-culture of virus-loaded (Ad-Track and LentiR.E1A) HUMSCs and HepG2 with different ratios in three independent samples.