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. 2016 Jun 17;7(32):51815–51828. doi: 10.18632/oncotarget.10122

Figure 4. Specific tumor suppressing effects of Ad-hTERTp-IL24 and low dose of 5-Fu could enhance growth inhibition of HepG2 cells.

Figure 4

A. The cell viability of HepG2 cells was measured every 24 hours after infection with different adenoviruses at MOI 100 by CCK8 assays. The results were expressed as survival rate of untreated control.** P<0.01. B. The cell viability of MRC-5 cells was measured every 24 hours after infection with different adenoviruses at MOI 100 by CCK8 assays. C. The inhibitory rate curve of HepG2 cells treated with 5-Fu gradient concentrations was analyzed. D. The survival rates of HepG2 cells infected by adenoviruses at 100 MOI in the presence or absence of low doses of 5-Fu were tested by CCK8 assay and expressed as mean ± SD. *P<0.05, ** P<0.01 compared with 5-Fu treatment group; #P<0.05, ##P<0.01 compared with Ad-hTERTp-IL24 treatment group. E. Apoptosis ratios were detected when 5-Fu in combination with Ad-hTERTp-IL24 or AdTrack and expressed as mean ± SD. *P<0.05, ** P<0.01 compared with 5-Fu treatment group; #P<0.05, ##P<0.01 compared with Ad-hTERTp-IL24 treatment group. (F) Western blot showed proteins involved in apoptosis pathway when 5-Fu in combination with Ad-hTERTp-IL24 or AdTrack. All the data represent the averages of three independent experiments.