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. 2016 Jun 17;7(32):51840–51853. doi: 10.18632/oncotarget.10123

Figure 7. PAUF exerts its role in human MDSCs.

Figure 7

The PAUF-mediated immunosuppressive function of human MDSCs was evaluated by measuring arginase A. NO B. and ROS C. production by rPAUF-treated or untreated MDSCs in the presence or absence of PMAb83 (15 μg/ml). D. Phosphorylation of ERK by PAUF, detected by flow cytometry, was also observed in rPAUF-treated human MDSCs. E. In vivo MDSC migration was evaluated by subcutaneous injection of PANC-1/Mock or PANC-1/PAUF cells into both flanks of NOD/SCID mice (n = 5), followed by adoptive transfer of DiR-labeled MDSCs isolated from human PBMCs after two weeks of tumor challenge, and bioluminescent imaging of tumor-infiltrated MDSCs 24 hours after the adoptive transfer. Data represent mean ± S.D. of three independent experiments, and representative results are shown. *, p < 0.05; **, p < 0.01.