Table 2. Effect of TKTL1 knock down on phospholipid synthesis in THP-1 cells.
| Species | Chain length (R) | THP-1WT | THP-1KD |
|---|---|---|---|
| Phosphatidyl-cholines (PC) | 31:0 | 369 ± 24 | 438 ± 11.5 |
| 32:2 | 285 ± 44 | 173 ± 29.8 | |
| 32:1 | 3533 ± 463 | 3008 ± 243 | |
| 32:0 | 1131 ± 67 | 888 ± 150 | |
| 33:3 | 64.1 ± 11.6 | 30.1 ± 4.1 | |
| 34:4 | 147 ± 20.9 | 172 ± 4.7 | |
| 34:4● | 15.7 ± 2,8 | 9.33 ± 0.4 | |
| 34:3 | 53.3 ± 5.0 | 44.8 ± 8.3 | |
| 34:2 | 1422 ± 167 | 1249 ± 74.8 | |
| 34:1 | 3624 ± 861 | 3362 ± 604 | |
| 35:4 | 116 ± 18 | 55.8 ± 4.0 | |
| 35:2 | 99.7 ± 29.8 | 59.2 ± 8.4 | |
| 35:1 | 306 ± 45 | 208 ± 24.1 | |
| 36:6 | 9.64 ± 2.8 | 4.54 ± 1.2 | |
| 36:5 | 37.0 ± 9.0 | 20.0 ± 5.0 | |
| 36:4 | 147 ± 29 | 91.2 ± 14.4 | |
| 36:3 | 356 ± 53 | 236 ± 25 | |
| 36:2 | 1543 ± 187 | 1128 ± 241 | |
| 36:1 | 1282 ± 128 | 1302 ± 115 | |
| 37:0 | 5.24 ± 1.3 | 6.32 ± 2.6 | |
| 38:8 | 1.40 ± 0.6 | 0.74 ± 0.1 | |
| 38:7 | 4.67 ± 0.7 | 2.20 ± 0.2 | |
| 38:6 | 52.2 ± 17.5 | 33.0 ± 9.2 | |
| 38:5 | 101 ± 16 | 44.3 ± 2.5 | |
| 38:4 | 70.2 ± 11 | 48.7 ± 3.8 | |
| 38:4● | 72.2 ± 7.6 | 93.9 ± 18.3 | |
| 38:3 | 97.8 ± 5.1 | 51.1 ± 16.0 | |
| 38:3● | 34.2 ± 8.2 | 17.3 ± 3.7 | |
| 38:2 | 350 ± 114 | 120 ± 98.3 | |
| 38:0 | 8.33 ± 4.2 | 7.28 ± 1.4 | |
| 40:7 | 22.1 ± 10.9 | 11.1 ± 6.0 | |
| 40:6 | 9.94 ± 4.0 | 6.67 ± 1.9 | |
| 40:6● | 12.7 ± 6.5 | 9.72 ± 1.7 | |
| 40:5 | 30.4 ± 17.8 | 17.0 ± 7.5 | |
| 40:2 | 27.1 ± 3.8 | 33.4 ± 3.4 | |
| 40:2● | 59.6 ± 3.3 | 36.4 ± 5.0 | |
| 42:2 | 75.5 ± 5.3 | 49.4 ± 2.1 | |
| 42:2● | 31.5 ± 4.1 | 22.7 ± 4.1 | |
| 44:3 | 17.7 ± 9.2 | 8.80 ± 2.7 | |
| 44:2 | 33.4 ± 24.1 | 9.99 ± 0.6 | |
| 44:2● | 14.8 ± 9.0 | 13.1 ± 2.5 | |
| 44:1 | 64.5 ± 44.5 | 48.4 ± 7.7 | |
| 46:1 | 5.03 ± 3.1 | 4.48 ± 1.6 | |
| TOTAL | 15033 ± 1319 | 12481 ± 560 ** | |
| Phosphatidyl-choline-plasmalogens (PC-pmg) | 34:2 | 16.7 ± 4.3 | 30.3 ± 6.5 |
| 34:1 | 135 ± 12 | 171 ± 21 | |
| 34:0 | 1212 ± 139 | 1415 ± 137 | |
| 36:4 | 37.5 ± 7.5 | 36.6 ± 2.9 | |
| 36:3 | 11,7 ± 4.5 | 23.2 ± 3.0 | |
| 36:3● | 33.1 ± 5.7 | 45.9 ± 7.9 | |
| 36:2 | 53.9 ± 0.7 | 25.6 ± 0.5 | |
| 36:2● | 46.7 ± 5.0 | - | |
| 36:1 | 138 ± 5.7 | 77 ± 55 | |
| 36:1● | 57.9 ± 13.5 | 14.8 ± 3.2 | |
| 36:0 | 263 ± 38 | 204 ± 97 | |
| 38:6 | 231 ± 33 | 179 ± 5.8 | |
| 38:5 | 29.9 ± 7.9 | 30.4 ± 5.8 | |
| 40:7 | 9.68 ± 2.6 | 6.00 ± 1.4 | |
| 40:6 | 18.4 ± 1.0 | 7.77 ± 1.8 | |
| TOTAL | 1985 ± 255 | 2073 ± 454 | |
| Lyso-phospatidyl-cholines (LPC) | 16:1 | 204 ± 38 | 154 ± 23 |
| 16:0 | 570 ± 154 | 579 ± 255 | |
| 18:3 | 100 ± 9 | 67.3 ± 13.6 | |
| 18:2 | 46.8 ± 6.6 | 39.9 ± 9.7 | |
| 18:1 | 352 ± 44 | 275 ± 39 | |
| 18:0 | 82.0 ± 14.6 | 124 ± 45 | |
| 20:4 | 85.0 ± 15.2 | 60.6 ± 16.7 | |
| 20:3 | 12.2 ± 0.8 | 15.2 ± 3.5 | |
| 20:1 | 19.5 ± 6.2 | 11.3 ± 2.3 | |
| 20:0 | 2.25 ± 0.1 | 1.79 ± 0.2 | |
| TOTAL | 1373 ± 175 | 1246 ± 281 | |
| Lyso-phospatidyl-choline-plasmalogens (LPC-pmg) | 16:1 | 16.7 ± 4.9 | 7.93 ± 3.4 |
| 16:0 | 80.6 ± 29.9 | 130.7 ± 41 | |
| 18.1 | 10.5 ± 2.4 | 12.3 ± 2.3 | |
| 18:0 | 10.4 ± 2.7 | 6.5 ± 1.2 | |
| TOTAL | 109 ± 25 | 153 ± 37 * | |
| Phosphatidyl-ethanolamines (PE) | 32:2 | 18.8 ± 7.3 | 11.9 ± 7 |
| 32:1 | 125 ±25 | 88,2 ± 28.6 | |
| 32:0 | 7.49 ± 0,6 | 4.84 ± 1.0 | |
| 34:3 | 17.1 ± 6.1 | 9.98 ± 4.6 | |
| 34:2 | 126 ± 13 | 70.1 ± 10.2 | |
| 34:1 | 165 ± 21 | 126 ± 9.7 | |
| 36:5 | 40.7 ± 25.3 | 29.2 ± 3.1 | |
| 36:4 | 45.7 ± 7.7 | 26.9 ± 7.4 | |
| 36:3 | 79.4 ± 8.1 | 35.4 ± 10.1 | |
| 36:2 | 165 ± 29 | 84.5 ± 6.0 | |
| 36:1 | 100 ± 13 | 80.4 ± 8.8 | |
| 38:7 | 51.8 ± 3.7 | 43.9 ± 1.7 | |
| 38:6 | 31.2 ± 6.4 | 16.0 ± 2.3 | |
| 38:5 | 42.0 ± 11.9 | 20.4 ± 6.1 | |
| 38:4 | 65.6 ± 7.9 | 36.4 ± 6.7 | |
| 38:2 | 13.0 ± 2.6 | 7.3 ± 1.6 | |
| 38.1 | 3.63 ± 0.2 | 1.79 ± 0.1 | |
| 38:1● | 13.5 ± 0.7 | 12.9 ± 0.5 | |
| 40:5 | 40.3 ± 9.4 | 24.7 ± 2.0 | |
| TOTAL | 1069 ± 94 | 660 ± 81 *** | |
| Phosphatidyl-ethanolamine-plasmalogens (PE-pmg) | 34:3 | 25.2 ± 2.9 | 19.9 ± 4.2 |
| 34:2 | 267 ± 65 | 210 ±15 | |
| 34:1 | 354 ± 61 | 268 ± 35 | |
| 36:4 | 141 ± 36 | 131 ± 9 | |
| 36:4● | 178 ± 5 | 159 ± 7 | |
| 36:3 | 67.9 ± 5.6 | 55.5 ± 2.9 | |
| 36:3● | 69.2 ± 7.2 | 38.0 ± 2.3 | |
| 36:2 | 84.4 ± 49.1 | 47.5 ± 24.0 | |
| 36:2● | 97.6 ± 13.9 | 46.0 ± 3.2 | |
| 36:1 | 102 ± 41 | 62.8 ± 6.2 | |
| 36:1● | 100 ± 6 | 51.7 ± 3.2 | |
| 36:0 | 36.4 ± 9.8 | 26.0 ± 1.8 | |
| 38:6 | 72.9 ± 14.5 | 70.2 ± 1.9 | |
| 38:6● | 113 ± 11 | 105 ± 5 | |
| 38:5 | 71.0 ± 11 | 84.4 ± 4.3 | |
| 38:5● | 64.0 ± 1.9 | 57.8 ± 1.9 | |
| 38:4 | 37.3 ± 10.3 | 17.3 ± 2.1 | |
| 40:7 | 0.26 ± 0.45 | 0.73 ± 0.23 | |
| 40:6 | 7.22 ± 0.6 | 4.25 ± 0.2 | |
| TOTAL | 1361 ± 220 | 1030 ± 89 * | |
| Lyso-phosphatidyl-ethanolamines (LPE) | 16:1 | 18.8 ± 7.3 | 12.8 ± 2.6 |
| 16:0 | 30.7 ± 8.1 | 27.6 ± 4.2 | |
| 18:2 | 15.6 ± 5.1 | 9.78 ± 1.5 | |
| 18:1 | 64.6 ± 24.7 | 36.7 ± 6.2 | |
| 18:0 | 44.4 ± 12.5 | 41.5 ± 4.1 | |
| 20:4 | 10.5 ± 3.2 | 6.25 ± 0.6 | |
| 20:3 | 8.1 ± 3.7 | 5.2 ± 2.3 | |
| TOTAL | 193 ± 59 | 140 ± 11 * | |
| Lyso-phosphatidyl-ethanolamine-plasmalogens (LPE-pmg) | 16:1 | 0.26 ± 0.07 | 0.24 ± 0.01 |
| 16:0 | 84.7 ± 9.8 | 83.0 ± 4.4 | |
| 18:1 | 5.13 ± 1.7 | 2.98 ± 0.7 | |
| 18.0 | 22.3 ± 5.4 | 20.3 ± 1.5 | |
| 20:1 | 0.55 ± 0.04 | 0.44 ± 0.07 | |
| 20:0 | 1.76 ± 1.0 | 1.49 ± 0.4 | |
| 22:1 | 1.01 ± 0.1 | 0.74 ± 0.04 | |
| 22:0 | 1.13 ± 0.1 | 0.89 ± 0.07 | |
| TOTAL | 66.8 ± 52.5 | 61.3 ± 45.4 | |
| Phospatidyl-serines (PS) | 32:1 | 34.0 ± 6.1 | 59.2 ± 1.4 |
| 34:2 | 13.3 ± 1.7 | 14.5 ± 7.9 | |
| 34:1 | 157 ± 43 | 172 ± 98 | |
| 36:5 | 25.0 ± 0.6 | 22.8 ± 0.9 | |
| 36:2 | 77.7 ± 8.3 | 56.3 ± 28.7 | |
| 36:1 | 218 ± 26 | 167 ± 66 | |
| 38:3 | 41.3 ± 8.0 | 57.7 ± 23.0 | |
| 38:2 | 23.7 ± 1.8 | 7.16 ± 0.7 | |
| 38:1 | 18.2 ± 1.4 | 5.16 ± 0.2 | |
| 40:6 | 48.2 ± 5.5 | 54.1 ± 0.5 | |
| 40:3 | 3.26 ± 0.3 | 2.14 ± 0.3 | |
| 40:2 | 12.0 ± 0.6 | 3.02 ± 0.2 | |
| 40:1 | 31.8 ± 8.9 | 22.4 ± 14.1 | |
| TOTAL | 621 ± 59 | 572 ± 276 | |
| Lyso-phospatidyl-serines (LPS) | 16:1 | 6.77 ± 1.6 | 12.8 ± 1.1 |
| 16:0 | 3.35 ± 1.4 | 4.70 ± 3.6 | |
| 18:3 | 12.8 ± 3.8 | 9.93 ± 4.7 | |
| 18:2 | 27.2 ± 10.1 | 27.1 ± 17.0 | |
| 18:1 | 28.3 ± 7.0 | 24.5 ± 15.9 | |
| 18.0 | 60.2 ± 22.0 | 67.8 ± 45.7 | |
| 20:4 | 14.5 ± 3.7 | 11.0 ± 6.4 | |
| 20:3 | 43.7 ± 6.6 | 31.8 ± 15.7 | |
| 20:2 | 30.0 ± 4.3 | 34.3 ± 20.2 | |
| 22:1 | 0.65 ± 0.0 | 0.32 ± 0.19 | |
| 22:0 | 2.72 ± 0.23 | 0.14 ± 0.25 | |
| TOTAL | 229 ± 51 | 220 ± 132 | |
| Phosphatidyl-inositols (PI) | 34:1 | 795 ± 178 | 708 ± 157 |
| 34:0 | 47.0 ± 21.8 | 64.3 ± 32.1 | |
| 36:4 | 152 ± 54 | 187 ± 69 | |
| 36:3 | 128 ± 15 | 110 ± 20 | |
| 36:2 | 697 ± 45 | 347 ± 53 | |
| 36:1 | 804 ± 109 | 397 ± 34 | |
| 38:4 | 441 ± 119 | 259 ± 122 | |
| 38:3 | 306 ± 25 | 114 ± 11 | |
| 40:6 | 462 ± 24 | 279 ± 24 | |
| TOTAL | 3452 ± 121 | 2257 ± 262 *** |
Values are expressed as pmol/106 cells (mean±SD; n=7; p-values are adjusted using a Holm method, *p<0.05, **p<0.01, ***p<0.001). To calculate the totals ± standard deviations per lipid species, we first calculated per repeat the sums of all chain length values, and then calculated the average and standard deviation of these sums. Due to the fact that in this high-throughput analysis, a peak was not present for each chain length in every repeat, some totals listed do not equal the mathematical sum of the individual chain length values.
R, total number of carbons: total number of insaturated acyl moieties.
●
Some species are detected by two independent peaks in the MS.