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. 2016 Jun 21;7(32):51955–51964. doi: 10.18632/oncotarget.10192

Figure 1. ST6Gal-I knockdown induces apoptosis and increases the sensitivity of MHCC97-H cells to docetaxel.

Figure 1

Ai-Aiii. ST6Gal-I was stably knocked down in MHCC97-H cells, the mRNA (Ai) and protein (Aii) levels of ST6Gal-I were determined by RT-PCR and Western-blot assays and normalized for GAPDH (*P<0.05). The α2,6-linked sialic acid (Aiii) levels were determined by SNA lectin staining. Coomassie Brilliant Blue (CBB) staining was used to normalize the protein amounts. B. The rates of apoptosis were determined by flow cytometry analysis of Annexin V-FITC/PI. Results are representative of three independent experiments (*P<0.05). C. Representative images of DAPI staining. Results are representative of 10 different fields (×100) from three independent experiments (*P<0.05). D. Cell viability following docetaxel treatment was detected by CCK8 assay. Note that the (1, 10, 100μM for 24h and 0.1, 1, 10, 100μM for 48h) measurements were significantly different (*P<0.05). Results represent the mean +/− SD of triplicate wells and are representative of at least three independent experiments. Par., MHCC97-H; shNC, Negative Control-shRNA transfectants; shST1 and shST2, ST6Gal-I-shRNA transfected stable clones.