Skip to main content
. 2016 Jul 7;7(32):52003–52016. doi: 10.18632/oncotarget.10468

Figure 5. MMP2 and MMP9 activity in cells overexpressing OPN-SV.

Figure 5

Gelatin zymography assay was used to analyze the levels of active MMP2 and MMP9 in conditioned medium from A. c643 (overexpressing OPNa, OPNb, OPNc and EV) and from B. 8505c (overexpressing OPNa, OPNb, OPNc and EV) cell clones. Representative images are shown. On the left are depicted molecular weights of standard markers; on the right are shown the variants of metalloproteinases (MMPs) observed in the gels. C. The intensity of gelatin-digested bands by pro- MMP2, MMP2 and MMP9 were measured by densitometry and are represented by the diagram bar. Percentage (%) of proteolytic activity from 8505c and c643 cells overexpressing OPNa, OPNb or OPNc was compared with the activity present in culture medium from 8505c and c643 control cells (EV). Data correspond to mean values of two independent experiments.