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. 2016 Jul 8;7(32):52032–52044. doi: 10.18632/oncotarget.10496

Figure 2. sTLR4/MD-2 complex restrains the binding of LPS to cellular membrane TLR4.

Figure 2

A. PMA-induced THP-1 cells or B. SW480 cells were treated with PBS, Isotype (FITC-IgG), LPS-FITC (1.0 μg/ml), LPS + LPS-FITC (both 1.0 μg/ml), LPS-FITC + sTLR4 (LPS-FITC 1.0 μg/ml + sTLR4 5.0 μg/ml), LPS-FITC + MD-2 (LPS-FITC 1.0 μg/ml + MD-2 1.25 μg/ml) or LPS-FITC + sTLR4/MD-2 (LPS-FITC 1.0 μg/ml + sTLR4/MD-2 6.25 μg/ml), respectively. The fluorescence intensity of cells in each group was determined by flow cytometry. Representative images of three independent experiments were shown.