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. 2016 Jul 7;7(32):52061–52084. doi: 10.18632/oncotarget.10474

Figure 8. Abrogation of hypoxia-induced invasion by CREB silencing.

Figure 8

The influence of 20 h hypoxia or normoxia on the migration A. and invasion B. of HER-2/neu− and HER-2/neu+ cells was analysed using trans-well inserts as described in Material and Methods. The bar charts represent three independent experiments performed in duplicates. C. The MMP activity under normoxic and hypoxic conditions in the culture supernatant was determined by gelatin zymography (left). Cells were incubated for 24 h under normoxic conditions (red) and were then incubated for the indicated time under hypoxia (blue) or were left under normoxia (green). The increased activity of MMP-9 and MMP-2 after 12 h or 24 h hypoxia is visible in both right lanes compared to the normoxic controls (left lanes). Parental and CREB-deficient HER-2/neu+ cells were incubated for 72 h under hypoxia and 20 μl supernatant was analysed on gelatin zymogram (right). A decreased activity of MMP-9 and of the cleaved MMP-2 was detected in CREB deficient cells, while the inactive MMP-2 (72 kDa) is not altered. The gels represent one of two independent experiments.