Table 1. Influence of CREB knock down on the expression of surface and angiogenesis markers.
| NIH3T3 | NIH3T3 shCREB | HER-2/neu+ | HER-2/neu+ shCREB | |
|---|---|---|---|---|
| Angiopoietin 1 | 1 | 0.56 +/− 0.23 | 4.06 +/− 3.01 | 4.63 +/− 0.41 |
| Angiopoietin 2 | 1 | 1.7 +/− 0.39 * | 0.65 +/− 0.7 | 1.45 +/− 0.44 |
| ICAM-1 | 1 | 0.82 +/− 0.5 | 0.12 +/− 0.03 | 2.07 +/− 1.2 * |
| PECAM1 | 1 | 1.08 +/− 0.54 | 0.28 +/− 0.34 | 0.29 +/− 0.23 |
| VCAM1 | 1 | 0.56 +/− 0.49 | 3.62 +/− 5.63 | 0.18 +/− 0.02 |
| VEGF-A | 1 | 0.87 +/− 0.53 | 2.76 +/− 0.31 | 0.79 +/− 0.49 * |
Cellular RNA isolated from parental and CREB-deficient (shCREB) HER-2/neu− and HER-2/neu+ cells was subjected to qPCR analysis as described in Materials and Methods. Using gene-specific primers the expression of two angiogenesis markers (VEGF, angiopoetin 1) as well as two adhesion molecules linked with angiogenesis (ICAM-1, VCAM1) was determined. The results represent the mean and SEM from three independent replicates. The expression was normalized to β-actin and the data are expressed as relative mRNA expression by setting the expression levels of NIH3T3 cells to 1.