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. 2016 Jul 13;7(32):52294–52306. doi: 10.18632/oncotarget.10561

Figure 4. The effect of SNAIL on TGF-β mediated M2-like polarization in THP-1 macrophages.

Figure 4

THP-1 macrophages transfected with siNC or siSNAIL were stimulated with or without TGF-β for 48 h. a. Expression of SNAIL was examined by qRT-PCR and western blot. b. The mRNA levels of MCP-1, CD80, TNF-α and CXCR4 in treated macrophages were detected by qRT-PCR. c. FACS analysis of CD86 and CD80 expression on siRNA transfected macrophages after treatment with (right panel) or without TGF-β (left panel). Macrophages transfected with SNAIL siRNA: unfilled histogram; macrophages transfected with negative control siRNA: closed histograms. d. ELISA analysis of the secretion of TNF-α and IL-12 by macrophages transfected with siRNA either in the presence of TGF-β or not. * p < 0.05 and ** p < 0.01 compared with control. Results represent mean ± SD of three separate experiments.