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. 2016 Jul 20;7(32):52392–52403. doi: 10.18632/oncotarget.10721

Figure 1. The effects of SREBP inhibition on gefitinib sensitivity.

Figure 1

A. A549 and PC9 cells were treated with indicated SREBP inhibitor (betulin 4 ug/mL, fatostatin 4 ug/mL and 25-HC 4 ug/mL) alone or combined with gefitinib (A549 10μM and PC9 5μM) for 48h. Relative cell viability was measured and calculated as described in “Materials and Methods”. Treatments by inhibitor combined with gefitinib have greater antiproliferative effect than each drug alone in both A549 and PC9 cells. B. A549 cells were transfected with NC-siRNA or SREBP1-siRNA. Two days after transfection, the protein levels of SREBP1 were examined by western blot, C. The mRNA levels of SREBP1 were examined by quantitative RT-PCR (C). D. The transfected A549 cells were treated with indicated concentration of gefitinib for 48h. Relative cell viability was measured and calculated as above. E. A549 cells were transfected with NC-siRNA or EGFR-siRNA. Two days after transfection, the protein levels of SREBP1 were examined by western blot. F. The transfected A549 cells were treated with indicated concentration of betulin for 48h. Relative cell viability was measured and calculated as above. G. A549 cells were treated with indicated EGFR TKI (afatinib 5μM, erlotinib 20μM) alone or combined with betulin (4 ug/mL). Relative cell viability was measured and calculated as above. Data are means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.005 by t-test.