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. 2016 Jul 20;7(32):52392–52403. doi: 10.18632/oncotarget.10721

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Copyright: © 2016 Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. HEK293T cells were co-transfected with Flag-SREBP1a and HA-MARVELD1. After 48 h of incubation, cell lysates were prepared in IP buffer and Flag-tagged proteins were immunoprecipitated with anti-Flag antibody (IgG as control). The presence of Flag- or HA-tagged proteins were analyzed by immunoblotting using anti-HA or anti-Flag antibody. B. HEK293T cell were transfected with HA-MARVELD1. After 48h, cell lysates were incubated with GST-fusion proteins of the indicated fragments of SREBP1a in GST pulldown assays. GST alone was a negative control. Bound HA-MARVEDL1 proteins were analyzed by immunoblotting using anti-HA antibody. C. Effects of MARVELD1 interference (or siRNA-NC as the control) on the SREBP target gene expression in A549 cells were analyzed by q-PCR. D. Effects of MARVELD1 interference on the SREBP transcriptional activity in A549 cells were analyzed using the Dual Luciferase assay system. E. Effects of MARVELD1 interference on the SREBP protein level. F. Chromatin immunoprecipitation (ChIP) assay was performed using antibodies against SREBP-1, MARVELD1 and IgG. Sequences were amplified by qPCR.