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. 2016 Jul 20;7(32):52392–52403. doi: 10.18632/oncotarget.10721

Figure 5. Effect of lipogenesis inhibition on cell membrane fluidity and EGFR phosphorylating activity.

Figure 5

A. The fluorescence excitation spectrum of DPH was examined in A549 cells treated with indicated fatty acid. B. Effect of MRVELD1 interference on the amounts of DPH bound to A549 cells. C. Effect of SREBP interference on the amounts of DPH bound to A549 cells. D. Betulin incubation markedly reduced the amounts of DPH bound to A549 cells and was partially rescued by oleic acid. E. A549 cells were incubated with for indicated concentration of betulin, cell lysates were prepared and analyzed by immunoblotting using the indicated antibodies. F. A549 cells were transfected with siRNA-SREBP (or siRNA-NC as the control). Samples were analyzed 48 h after transfection and the levels of actin were used as the loading control for immunoblotting. G. A549 cells were treated with indicated concentration of oleic acid. After 4h of incubation, cell lysates were prepared and analyzed by immunoblotting using the indicated antibodies. H. Effect of betulin on gefitinib sensitivity with or without oleic acid was examined as above. Data are means ± SD (n = 3). *P < 0.05.