Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 11;96(1):126–134. doi: 10.4269/ajtmh.16-0503

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The American Society of Tropical Medicine and Hygiene

PMC Copyright notice

Figure 1. — Expression of DENV-4 E in Pichia pastoris. (A) Schematic representation of DENV-4 E gene cloned in pPICZ-A expression vector with AOX1 promoter (5′ AOX1) upstream and transcription terminator sequence (TT) downstream of it. An Escherichia coli origin of replication (ori) and zeocin resistance (Zeo) marker is also present for efficient replication and transformed clone selection, respectively. (B and C) Evaluation of expression and localization of DENV-4 E in induced P. pastoris. Aliquots of un-induced (UI) and induced (I) cell cultures were subjected to lysis and separated into soluble (S) and membrane-enriched pellet (P) fractions by centrifugation. S and P fractions were evaluated through (B) Western blot and (C) ELISA on Ni-NTA His Sorb plate using DENV-4 E-specific monoclonal antibody (mAb), DENV-4 E88. The blot in panel B is a composite figure, where lane “M” denotes pre-stained markers, the sizes (in kDa) of which are indicated on the left. An arrow on the right of panel B indicates the position of DENV-4 E in the blot. In panel C, the S and P fractions are represented by the grey and black bars, respectively. DENV-4 E = dengue virus serotype 4 ectodomain; ELISA = enzyme-linked immunosorbent assay.