Table 1.
Evaluation of epitope integrity of DENV-1, 2, 3, and 4 VLPs*
| Serotype-specific murine mAbs | ||||||
|---|---|---|---|---|---|---|
| mAb† | Epitope specificity | Absorbance at 450 nm | ||||
| 1E | 2E | 3E | 4E | HBsAg | ||
| DENV1 E103 | Anti-EDIII LR | 3.48 | 0.06 | 0.06 | 0.05 | 0.03 |
| DENV1 E24 | Anti-EDIII | 1.23 | 0.03 | 0.02 | 0.03 | 0.03 |
| DENV1 E29 | Anti-EDIII | 1.87 | 0.03 | 0.09 | 0.14 | 0.03 |
| DENV1 E37 | Anti-EDIII | 0.71 | 0.02 | 0.03 | 0.01 | 0.04 |
| DENV2 3H5 | Anti-EDIII LR | 0.14 | 3.46 | 0.06 | 0.11 | 0.03 |
| DENV2 104 | Anti-EDIII CC loop | 0.08 | 3.45 | 0.1 | 0.05 | 0.04 |
| DENV2 70 | Anti-EDIII AS/LR | 0.02 | 0.92 | 0.03 | 0.03 | 0.02 |
| DENV2 106 | Anti-EDIII LR | 0.05 | 2.5 | 0.03 | 0.03 | 0.05 |
| DENV3 8A1 | TS | 0.08 | 0.05 | 1.35 | 0.03 | 0.03 |
| DENV3 E3 | Anti-EDIII | 0.13 | 0.03 | 2.90 | 0.02 | 0.02 |
| DENV3 E1 | Anti-EDIII | 0.04 | 0.03 | 3.43 | 0.02 | 0.03 |
| DENV3 E4 | Anti-EDI/II | 0.12 | 0.04 | 3.18 | 0.03 | 0.03 |
| DENV4 E29 | Anti-EDIII | 0.04 | 0.04 | 0.03 | 2.80 | 0.02 |
| DENV4 E42 | Anti-E | 0.03 | 0.02 | 0.02 | 2.90 | 0.02 |
| DENV4 E43 | Anti-E | 0.02 | 0.02 | 0.02 | 2.15 | 0.03 |
| DENV4 E76 | Anti-EDIII | 0.05 | 0.07 | 0.03 | 2.32 | 0.04 |
| DENV4 E88 | Anti-EDIII LR | 0.02 | 0.03 | 0.02 | 2.94 | 0.03 |
| Cross-reactive murine and human mAbs | ||||||
| 4G2‡ | FL | 0.23 | 0.2 | 0.83 | 0.41 | 0.04 |
| 12 C1‡ | Anti-EDIII, not LR | 3.76 | 3.86 | 3.72 | 3.68 | 0.04 |
| h-1M7‡ | FL | 2.97 | 3.03 | 2.48 | 2.73 | 0.03 |
| h-23.13 | EDI/II | 0.23 | 0.1 | 0.52 | 0.26 | 0.03 |
| h-1N5‡ | FL | 0.95 | 0.45 | 1.47 | 1.06 | 0.05 |
| h-2J20 | Anti-EDIII | 3.67 | 3.46 | 3.58 | 0.27 | 0.02 |
| h-2K2 | prM | 0.07 | 0.06 | 0.06 | 0.06 | 0.05 |
AS = A strand; DENV = dengue virus; ED = envelope domain; ELISA = enzyme-linked immunosorbent assay; FL = fusion loop; LR = lateral ridge; mAb = monoclonal antibody; prM = pre-membrane; TS = type specific; VLP = virus-like particle.
Evaluated by indirect ELISA using Pichia Pastoris–expressed purified DENV E VLPs as coating antigens.
Serotype-specific mAbs used in ELISA: DENV-1 mAbs E103, E24, E29, and E3728; DENV-2 mAb 3H536; DENV-2 mAbs 104, 70, and 10638; DENV-3 mAb 8A140; DENV-3 mAbs E3, E1, and E437; DENV-4 mAbs E29, E42, E43, E76, and E88.39 Cross-reactive mAbs: 4G236; 12C140; h-1N5 and h-1M7,43 h-23.1341; h-2J2044; h-2k2. All mAbs are murine, except those that are prefixed with h-, which indicates human.
Complex-specific mAbs; 4G2, h-1N5, and h-1M7 bind the fusion loop of all four DENV-Es; 12 C1 binds recombinant EDIII (outside the LR epitope) of all four DENV serotypes.