Figure 2.

Testing of 50 clinical stool specimens using the combined polymerase chain reaction assay. The vertical (a) and (b) dashed lines show the consensus T_m peak values used to identify Opisthorchis viverrini (83.11 [±0.12]) and Haplorchis taichui (82.09 [±0.06]). (a1) and (b1) represent the detection limits of the O. viverrini and H. taichui COI gene plasmids, respectively. The letter O indicates the other templates that were not amplified by the two specific primers, including the negative control (no DNA template). In total, (A) 24 consensus T_m peaks were identified as O. viverrini, (B) 31 consensus T_m peaks were identified as H. taichui, and (C) 21 consensus O. viverrini T_m peaks and 21 consensus H. taichui T_m peaks were detected in the coinfected specimens. Furthermore, (D) 16 consensus T_m peaks were amplified by the O. viverrini– or H. taichui–specific primers in samples that were found to be negative for “Opisthorchis-like” eggs during microscopic examinations. Figures 2D-1 and 2D-2 show the amplification curves and T_m peak values for the samples in which O. viverrini (sample no. 35 in Table 3) and H. taichui (sample nos. 35, 36, 37, and 40 in Table 3) were detected, respectively.