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. 2017 Jan 11;96(1):221–228. doi: 10.4269/ajtmh.16-0165

Table 1.

Primers for PCR and real-time PCR SYBR Green I–based assays

No. Primer name Direction Nucleotide sequence (5′–3′) Length (bp) Product size (bp)
1 MCOI-A F TTT TTT GGG CAT CCT GAG GTT TA 23 444
2 MCOI-B R TAA AGA AAG AAC ATA ATG AAA ATG AGC 27
3 PKZ1613F F CTT CTT TGG TTA TGC GGG TTT AGT TC 26 218
4 PKZ1613R R TTA TCA GAT CCC AAA ACA GGC CC 23
5 QV301F F GTC ATA TCT GTA CGA CGT TCA CTG GT 26 155
6 QV301R R GCT AAA AAA CAC AGC GGT GCC 21

F = forward; R = reverse; PCR = polymerase chain reaction.