Figure 4. Analysis of body weight, temperature, paw edema and mRNA expression of select cytokines and growth factors in skin after GH pretreatment in mice after P14 inflammation.

At P14, no differences in body weight were observed in any of the three groups (A) however, carrageenan injection induced an increase in body temperature that was similarly observed in the GH treatment group (B). * p<0.05 vs. naive; ^#p<0.05 vs. naïve, but not Carr.+GH treated mice; n=4–11/group; One-way ANOVA/Holm Sidak post hoc. Carrageenan induced paw edema was not affected by a GH pretreatment regimen (C). Only IL1β was upregulated in the hairy hindpaw skin of P14 inflamed neonates (D). GH treated neonates with cutaneous inflammation also showed upregulation of this cytokine in the skin. Although both groups showed significant upregulation of IL1β in the skin after inflammation, there were no statistically significant differences between these groups in regards to relative gene expression. Yellow highlighted values indicate p<0.05 vs. naïve only; n=3–7 for C, D. undet= undetectable. One-way ANOVA/Tukey’s post hoc. Values are presented as a percent change from naïve.