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Principle of GO-QDs FRET biosensor. In the system, carboxyl QDs (donor) and GO (quencher) were first conjugated with the capture probes B and A, respectively, with the aid of EDC/NHS. Upon the addition of the complementary invA oligo of Salmonella, the QD and GO conjugates could be brought into close proximity to make the FRET pair work, the energy emitted from excited QDs would be quenched by GO.
