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. 2017 Jan 17;8:8. doi: 10.3389/fmicb.2017.00008

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Copyright © 2017 Guo, Chan, Chen and Zeng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The specificity of the developed invA gene biosensor. The concentrations of invA oligo (fully complementary to probes), M1 (one-base mismatch), M2 (two-base mismatch) and control oligo (not complementary to neither probes) used were fixed at 400 nM. The SEM (Standard Error of the Mean) error bars were calculated from at least three replicates. The difference between invA oligo and control oligo groups was significant (P < 0.0001, two tail t-test), but the difference between invA oligo and M1 or M2 groups were not significant (P > 0.05, two tail t-test). The data was analyzed by GraphPad Prism. ^∗∗∗P < 0.0001.