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. 2017 Jan 17;7:2060. doi: 10.3389/fmicb.2016.02060

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Copyright © 2017 Wang, Xie, Zhu, Zhou and Lu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PCR verification of TYΔndrA1 and TYΔndrB2 using specific primers beyond the homologous fragments and primers in the resistance gene. (A) Sample 1, amplified with ndrA1-VF and Kan02, 2260 bp; sample 2, amplified with ndrA1-VR and Kan01, 2172 bp; sample 3, amplified with ndrB2-VF and Kan01, 2308 bp; sample 4, amplified with ndrB2-VR and Kan02, 2330 bp; M1, DL15000 marker; M2, DL2000 marker. Electrophoresis was performed using 0.8% agarose; (B) The in-frame deletion of target genes, and relative site of genomic-specific primers and resistance gene-specific primers.