Skip to main content
. 2017 Jan 17;4:100. doi: 10.3389/fbioe.2016.00100

Figure 1.

Figure 1

Nitric oxide production in Chlorella vulgaris detected by 4,5-diaminofluorescein staining. (A) C. vulgaris grown in Bold’s Basal Medium (BBM) with 3 mM NaNO3 (white bar) or treated with 1 mM NaNO2 in BBM pH 4.5 for 1 h (black bar) were stained with 4,5-diaminofluorescein to detect nitric oxide production fluorometrically. (B) Bright field (left panel) and fluorescence (right panel) microscopy of a representative NaNO2-treated sample.