Table 1.

Human blood fractions obtained in the investigation.

Blood fraction	Description	Method of isolation	Characteristics of the method
(I) cells	Whole blood, enriched with blood cells	1,200g∗, pellet	Preparation of platelet-poor plasma [19] and estimation of haemolysis [20]
(II) plasma	Platelet-poor blood plasma	1,200g∗, supernatant
(III) 16,000g plasma pellets	Intermediate fraction containing membrane vesicles and cell debris	16,000g∗, pellet	Isolation of microvesicles [21]
(IV) 160,000g plasma pellets	Fraction enriched with exosomes	160,000g∗∗, pellet	High-recovery isolation of exosomes [22]
(V) 160,000g plasma supernatant	Vesicle-depleted plasma	160,000g∗∗, supernatant

^∗Centrifugation for 20 min at 4°C.

^∗∗Centrifugation for 120 min at 4°C.