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. 2016 Nov 16;36(2):202–212. doi: 10.15252/embj.201694834

Figure EV2. Phosphorylation of T387 STAT2 inhibits the antiviral effect of IFN‐β in HME and U6A cells.

Figure EV2

  • A
    HME cells expressing wild‐type, T387A, or T387D STAT2 were seeded at 8,000 cells/well. The cells were exposed to VSV for 2 h, with or without pre‐treatment with IFN‐β (100 IU/ml). After 20 h, the expression of GFP from modified VSV was analyzed by microscopy.
  • B, C
    U6A cells expressing wild‐type or T387A STAT2 were seeded at 8,000 cells/well. The cells were exposed to VSV for 2 h, with or without pre‐treatment with IFN‐β (100 IU/ml). After 20 h, the expression of GFP from modified VSV was analyzed by microscopy (B) and the VSV‐M protein was analyzed by Western blot (C).
  • D
    U6A cells expressing wild‐type or T387A STAT2 were seeded at 8,000 cells/well. The cells were exposed to HSV for 2 h, with or without pre‐treatment with IFN‐β (100 IU/ml). After 20 h, the HSV ICP0 protein was analyzed by Western blot.