Figure EV4. Analysis of SSA by Southern blot hybridization in SRS2 and srs2Δ cells.

• A, B
  Schematics and a representative Southern blot images are shown for fragment S1 and S2 in panels (A) and (B), respectively (see Fig 5A for further explanation). In each schematic, a black triangle represents the HO site. Sites for the restriction enzymes used in the analysis of the corresponding DNA fragment are shown as vertical arrows. Hybridization probe spans the region of homology indicated by grey shadows. F1 and F2 correspond to fragments 1 and 2, respectively, indicated by two‐ended arrows at the bottom of each schematic; SSA, the product of repair (fragments S1 and S2 in panels A and B, respectively); REF, a fragment on chr.IV (ARS1 locus) detected by the reference probe; C, control strain. Numbers above each gel lane indicate time points in the time‐course experiments.