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. 2017 Jan 1;23(1):38–49. doi: 10.1089/ten.tec.2016.0299

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© Minu Karthika Ganesan et al., 2017; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 2. — The 3D SW-CC between days 1 and 7. (A) Phase contrast images, day 1 (D1): secretory SMC, day 4 (D4): contractile SMC, day 5 (D5): EC added on top of SMC, and day 7 (D7): EC monolayer on top of contractile SMC, scale bar = 200 μm. (B) Western blot analysis for cyclin D1, calponin 1, and VE-cadherin protein expression at indicated days. β-actin served as loading control. Band intensities were normalized to β-actin. Bar graphs display mean ± SD. n = 4, *p < 0.05, one way ANOVA with Tukey's multiple comparison post hoc test. ANOVA, analysis of variance; SD, standard deviation; VE, vascular endothelial.