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. 2017 Jan 1;23(1):38–49. doi: 10.1089/ten.tec.2016.0299

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© Minu Karthika Ganesan et al., 2017; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

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FIG. 3. — Three-dimensional reconstruction of the 3D SW-CC on day 7. (A) Confocal 3D reconstruction of the 3D SW-CC showing two separated cell layers. Before seeding, SMC were stained red with cell tracker^™ orange and EC were stained green with cell tracker green. (B) Orthogonal cut through a Z stack gallery showing EC (loaded with cell tracker green) residing on top of SMC (loaded with cell tracker orange). (C) Representative confocal images from a Z stack gallery at indicated distances from the bottom of the well. The 3D SW-CC was fixed and stained for α-smooth muscle actin (red) for SMC and VE-cadherin (green) for EC, scale bar = 50 μm. (D) 3D reconstruction of a Z stack gallery. Left image: lateral view, right image: anterior view. The 3D SW-CC was fixed and stained for α-smooth muscle actin (red) for SMC and VE-cadherin (green) for EC, scale bar = 50 μm.