FIG. 7.

Stimulation of the 3D SW-CC with activated platelets. Representative confocal images of the 3D SW-CC from a Z stack gallery at indicated distances from the bottom of the well shown along with the respective orthogonal cuts of (A) control 3D SW-CC on day 7, (B) 3D SW-CC after coculturing with platelets (ratio of 100:1) for 24 h. Following fixation, cells were stained for α-SMA (red), VE-cadherin (green), and CD41 for platelets (white). Scale bar = 50 μm. (C) At indicated time points following the addition of platelets, cells were lysed, mRNA was isolated, and expression of E-selectin and ICAM-1 was determined by quantitative real-time PCR in indicated cultures. Expression levels were normalized to the respective unstimulated condition (0 h). Bar graphs display mean ± SD. n = 4, *p < 0.05, two-way ANOVA with Bonferroni post hoc test.