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. 2017 Jan 17;7:2. doi: 10.3389/fcimb.2017.00002

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Copyright © 2017 Zakharzhevskaya, Tsvetkov, Vanyushkina, Varizhuk, Rakitina, Podgorsky, Vishnyakov, Kharlampieva, Manuvera, Lisitsyn, Gushina, Lazarev and Govorun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Full mass spectra of B. fragilis lipids show the most represented lipids of B. fragilis membrane. (A) MS analysis of ETBF (colored in red) and NTBF (colored in blue) membrane in positive linear mode. (B) MS analysis of ETBF (colored in red) and NTBF (colored in blue) membrane in negative linear mode. B. fragilis cells membrane lipids were extracted by Folch method (see material and methods), dissolved in 200 μl ethanol and analyzed by quadruple time-of-flight tandem mass spectrometer. Mass spectrum of ETBF and NTBF in positive and negative linear mode showed the same cluster of peaks, indicating the most represented classes of lipids (PC, PE, DG, PA).