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. 2017 Jan 17;7:40534. doi: 10.1038/srep40534

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Histochemical analysis of phosphorylated ERK1/2 (pERK) (a; green), calcium sensing receptor (CaSR) (b; red), and vitamin D receptor (VDR) (c; red). Paraformaldehyde-treated paraffin-embedded thyro-parathyroid glands were sectioned and stained for pERK, CaSR, and VDR using the indirect immunofluorescence staining technique as described in the Methods section. Fgfr1–3^flox/flox, αKlotho^flox/flox, or Fgfr1–4^flox/flox mice were mated (cKO) or not mated (non-cKO) with PTH-Cre mice, and treated (heminephrectomy plus high-phosphate diet) or not treated for CKD, as described in the Methods section. Scale bars: 10 μm.